Peptide Library

Overlapping library

Overlapping peptide library is generated by breaking the original protein or peptide into many equal-length overlapping fragment and it is used for linear, continuous epitope mapping and T-cell epitope determination.

Example: RILCGSSWNVLDFEISGKLGEHVIVLVVAK(30AA protein)

Peptide length: 10 mer
Offset: 5
No. Peptide 5

 Alanine Library

Alanine Scanning Library: Generation of peptide library in which alanine(Ala,A) is systematically substituted into each of the amino acids in the identified peptide sequence (epitope)

Example:

 Positional library

Selected position in a peptide sequence is systematically replaced with different amino acids to determine the preferred amino acid residues at these positions, as manifested by corresponding increases in activity

Example:

Turncation Library

Truncation Library: Series of peptide sequences representing the systematic truncation of the flanking residues to determine the minimum length required for optimum peptide activity

Example:

Random library

Random Library is similar to positional scanning library. It’s constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity

Scramble peptide library

Scramble peptide library：Scramble Library is constructed by carrying out permutation on the original peptide’s sequence. It has the potential to give all possible alternatives and offers and represents the highest degree of variability for peptide library.

T-cell Truncated peptide library

T-cell Truncated peptide libraries：Allow the testing of all possible T-cell epitopes across a protein of interest